MALDI-2 and t-MALDI-2 Mass Spectrometry Imaging.
Dreisewerd K, Bien T, Soltwisch J
Research article (book contribution) | Peer reviewedAbstract
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) combined with laser-induced postionization for MALDI-2 enables the simultaneous registration of numerous classes of small molecules (e.g., secondary metabolites including sterols) as well as phospholipids, glycolipids, and glycans from tissue sections and from cell cultures with strongly boosted ion yields. Here, we describe methodological aspects that are key for optimizing the analytical sensitivity and spatial resolution of a MALDI-2 imaging experiment. We will include both top-illumination MALDI-2 as well as the recently introduced transmission (t-) mode MALDI-2 approach.
Details about the publication
Publisher: Lee, YJ.
Book title: Mass Spectrometry Imaging of Small Molecules (Volume 2437)
Page range: 21-40
Publishing company: Humana Press
Place of publication: New York, USA
Title of series: Methods in Molecular Biolog (ISSN: 1064-3745)
Volume of series: 2437
Status: Published
Release year: 2022 (14/12/2021)
Language in which the publication is written: English
ISBN: 978-1-0716-2029-8
Keywords: Diagnostic Imaging; Glycolipids; Lasers; Phospholipids; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; MALDI imaging MALDI-2 Transmission-mode MALDI-2 Sample preparation Cell cultures
Authors from the University of Münster
| Bien, Tanja | Institute of Hygiene |
| Dreisewerd, Klaus | Institute of Hygiene |
| Soltwisch, Jens | Institute of Hygiene |